Our expertise is intestinal lipid absorption, lipoprotein synthesis, secretion, and metabolism, and using the primary intestinal organoid model. 

Major efforts in the lab focus on:

 
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Lipids and Tregs

The mechanism of apoC-III regulation of intestinal regulatory T cells (Tregs) and the therapeutic implications of this mechanism in inflammatory bowel disease (IBD)

 
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intestinal lipid handling

Determining the influence of plasma lipoproteins, particularly triglyceride-rich lipoproteins with apoC-III, on intestinal lipid metabolism. Key questions include: How do plasma lipoproteins regulate enterocyte metabolism? Does this impact dietary lipid absorption? How does this influence inflammatory disease?

 
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intestinal organoids

Using this model system to determine mechanisms in human intestine.

Publications

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1: Li D, Dong H, Kohan AB. The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism. Methods Mol Biol. 2017 Sep 20. doi: 10.1007/7651_2017_69. [Epub ahead of print] PubMed PMID: 28929461.

2: Botteri G, Montori M, Gumà A, Pizarro J, Cedó L, Escolà-Gil JC, Li D, Barroso E, Palomer X, Kohan AB, Vázquez-Carrera M. VLDL and apolipoprotein CIII induce ER stress and inflammation and attenuate insulin signalling via Toll-like receptor 2 in mouse skeletal muscle cells. Diabetologia. 2017 Nov;60(11):2262-2273. doi: 10.1007/s00125-017-4401-5. Epub 2017 Aug 23. PubMed PMID: 28835988.

3: West G, Rodia C, Li D, Johnson Z, Dong H, Kohan AB. Key differences between apoC-III regulation and expression in intestine and liver. Biochem Biophys Res Commun. 2017 Sep 23;491(3):747-753. doi: 10.1016/j.bbrc.2017.07.116. Epub 2017 Jul 21. PubMed PMID: 28739253.

4: Jattan J, Rodia C, Li D, Diakhate A, Dong H, Bataille A, Shroyer NF, Kohan AB. Using primary murine intestinal enteroids to study dietary TAG absorption, lipoprotein synthesis, and the role of apoC-III in the intestine. J Lipid Res. 2017 May;58(5):853-865. doi: 10.1194/jlr.M071340. Epub 2017 Feb 3. PubMed PMID: 28159868; PubMed Central PMCID: PMC5408603.

5: Kohan AB, Yang Q, Xu M, Lee D, Tso P. Monosodium glutamate inhibits the lymphatic transport of lipids in the rat. Am J Physiol Gastrointest Liver Physiol. 2016 Oct 1;311(4):G648-G654. doi: 10.1152/ajpgi.00342.2014. Epub 2016 Aug 11. PubMed PMID: 27514481; PubMed Central PMCID: PMC5142199.

6: Kassis T, Yarlagadda SC, Kohan AB, Tso P, Breedveld V, Dixon JB. Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity. Am J Physiol Gastrointest Liver Physiol. 2016 May 15;310(10):G776-89. doi: 10.1152/ajpgi.00318.2015. Epub 2016 Mar 11. PubMed PMID: 26968208; PubMed Central PMCID: PMC4888550.

7: Wang F, Yoder SM, Yang Q, Kohan AB, Kindel TL, Wang J, Tso P. Chronic high-fat feeding increases GIP and GLP-1 secretion without altering body weight. Am J Physiol Gastrointest Liver Physiol. 2015 Nov 15;309(10):G807-15. doi: 10.1152/ajpgi.00351.2013. Epub 2015 Sep 3. PubMed PMID: 26336929; PubMed Central PMCID: PMC4652142.

8: Kohan AB. Apolipoprotein C-III: a potent modulator of hypertriglyceridemia and cardiovascular disease. Curr Opin Endocrinol Diabetes Obes. 2015 Apr;22(2):119-25. doi: 10.1097/MED.0000000000000136. Review. PubMed PMID: 25692924; PubMed Central PMCID: PMC4524519.

9: Wang F, Kohan AB, Lo CM, Liu M, Howles P, Tso P. Apolipoprotein A-IV: a protein intimately involved in metabolism. J Lipid Res. 2015 Aug;56(8):1403-18. doi: 10.1194/jlr.R052753. Epub 2015 Feb 1. Review. PubMed PMID: 25640749; PubMed Central PMCID: PMC4513983.

10: Kohan AB, Wang F, Lo CM, Liu M, Tso P. ApoA-IV: current and emerging roles in intestinal lipid metabolism, glucose homeostasis, and satiety. Am J Physiol Gastrointest Liver Physiol. 2015 Mar 15;308(6):G472-81. doi: 10.1152/ajpgi.00098.2014. Epub 2015 Jan 15. Review. PubMed PMID: 25591862; PubMed Central PMCID: PMC4360046.

11: Wang F, Kohan AB, Dong HH, Yang Q, Xu M, Huesman S, Lou D, Hui DY, Tso P. Overexpression of apolipoprotein C-III decreases secretion of dietary triglyceride into lymph. Physiol Rep. 2014 Mar 20;2(3):e00247. doi: 10.1002/phy2.247. Print 2014. PubMed PMID: 24760506; PubMed Central PMCID: PMC4002232.

12: Ohlsson L, Kohan AB, Tso P, Ahrén B. GLP-1 released to the mesenteric lymph duct in mice: effects of glucose and fat. Regul Pept. 2014 Feb 10;189:40-5. doi: 10.1016/j.regpep.2014.02.001. Epub 2014 Feb 28. PubMed PMID: 24583245; PubMed Central PMCID: PMC4054822.

13: Li X, Xu M, Wang F, Kohan AB, Haas MK, Yang Q, Lou D, Obici S, Davidson WS, Tso P. Apolipoprotein A-IV reduces hepatic gluconeogenesis through nuclear receptor NR1D1. J Biol Chem. 2014 Jan 24;289(4):2396-404. doi: 10.1074/jbc.M113.511766. Epub 2013 Dec 5. PubMed PMID: 24311788; PubMed Central PMCID: PMC3900982.

14: Kohan AB, Wang F, Li X, Vandersall AE, Huesman S, Xu M, Yang Q, Lou D, Tso P. Is apolipoprotein A-IV rate limiting in the intestinal transport and absorption of triglyceride? Am J Physiol Gastrointest Liver Physiol. 2013 Jun 15;304(12):G1128-35. doi: 10.1152/ajpgi.00409.2012. Epub 2013 Apr 18. PubMed PMID: 23599044; PubMed Central PMCID: PMC3680714.

15: Walsh CM, Suchanek AL, Cyphert TJ, Kohan AB, Szeszel-Fedorowicz W, Salati LM. Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver. J Biol Chem. 2013 Jan 25;288(4):2816-28. doi: 0.1074/jbc.M112.410803. Epub 2012 Dec 11. PubMed PMID: 23233666; PubMed Central PMCID: PMC3554946.

16: Kassis T, Kohan AB, Weiler MJ, Nipper ME, Cornelius R, Tso P, Dixon JB. Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function. J Biomed Opt. 2012 Aug;17(8):086005. doi: 10.1117/1.JBO.17.8.086005. PubMed PMID: 23224192; PubMed Central PMCID: PMC3413897.

17: Kohan AB, Howles PN, Tso P. Methods for studying rodent intestinal lipoprotein production and metabolism. Curr Protoc Mouse Biol. 2012 Sep 1;2:219-230. PubMed PMID: 23024926; PubMed Central PMCID: PMC3458698.

18: Kohan AB, Vandersall AE, Yang Q, Xu M, Jandacek RJ, Tso P. The transport of DDT from chylomicrons to adipocytes does not mimic triacylglycerol transport. Biochim Biophys Acta. 2013 Feb;1831(2):300-5. doi: 10.1016/j.bbalip.2012.07.020. Epub 2012 Aug 3. PubMed PMID: 22885168; PubMed Central PMCID: PMC3534790.

19: Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB. Activation of the farnesoid X receptor induces hepatic expression and secretion of fibroblast growth factor 21. J Biol Chem. 2012 Jul 20;287(30):25123-38. doi: 10.1074/jbc.M112.375907. Epub 2012 Jun 1. PubMed PMID: 22661717; PubMed Central PMCID: PMC3408207.

20: Wang F, Kohan AB, Kindel TL, Corbin KL, Nunemaker CS, Obici S, Woods SC, Davidson WS, Tso P. Apolipoprotein A-IV improves glucose homeostasis by enhancing insulin secretion. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9641-6. doi: 10.1073/pnas.1201433109. Epub 2012 May 22. PubMed PMID: 22619326; PubMed Central PMCID: PMC3386109.

21: Kohan AB, Wang F, Li X, Bradshaw S, Yang Q, Caldwell JL, Bullock TM, Tso P. Apolipoprotein A-IV regulates chylomicron metabolism-mechanism and function. Am J Physiol Gastrointest Liver Physiol. 2012 Mar 15;302(6):G628-36. doi: 10.1152/ajpgi.00225.2011. Epub 2011 Dec 29. PubMed PMID: 22207575; PubMed Central PMCID: PMC3311309.

22: Kohan AB, Yoder SM, Tso P. Using the lymphatics to study nutrient absorption and the secretion of gastrointestinal hormones. Physiol Behav. 2011 Nov 30;105(1):82-8. doi: 10.1016/j.physbeh.2011.04.056. Epub 2011 May 13. Review. PubMed PMID: 21605578; PubMed Central PMCID: PMC3179774.

23: Kohan AB, Qing Y, Cyphert HA, Tso P, Salati LM. Chylomicron remnants and nonesterified fatty acids differ in their ability to inhibit genes involved in lipogenesis in rats. J Nutr. 2011 Feb;141(2):171-6. doi: 10.3945/jn.110.129106. Epub 2010 Dec 15. PubMed PMID: 21169224; PubMed Central PMCID: PMC3021438.

24: Kohan A, Yoder S, Tso P. Lymphatics in intestinal transport of nutrients and gastrointestinal hormones. Ann N Y Acad Sci. 2010 Oct;1207 Suppl 1:E44-51. doi: 10.1111/j.1749-6632.2010.05753.x. Review. PubMed PMID: 20961305.

25: Yang L, Li X, Ji Y, Kohan AB, Wang DQ, Howles PN, Hui DY, Lai J, Tso P. Effect of ezetimibe on incretin secretion in response to the intestinal absorption of a mixed meal. Am J Physiol Gastrointest Liver Physiol. 2010 Nov;299(5):G1003-11. doi: 10.1152/ajpgi.00294.2010. Epub 2010 Jul 22. PubMed PMID: 20651007; PubMed Central PMCID: PMC2993164.

26: Kohan AB, Talukdar I, Walsh CM, Salati LM. A role for AMPK in the inhibition of glucose-6-phosphate dehydrogenase by polyunsaturated fatty acids. Biochem Biophys Res Commun. 2009 Oct 9;388(1):117-21. doi: 10.1016/j.bbrc.2009.07.130. Epub 2009 Jul 30. PubMed PMID: 19646964; PubMed Central PMCID: PMC2756033.